Dendritic Cells, Infected with Vesicular Stomatitis Virus-Pseudotyped HIV-1, Present Viral Antigens to CD4+and CD8+T Cells from HIV-1-Infected Individuals

Dendritic cells, infected with vesicular stomatitis virus-pseudotyped HIV-1, present viral antigens to CD4+ and CD8+ T cells from HIV-1-infected individuals.

Nonreplicating vectors are being considered in HIV-1 vaccine design. However, nonreplicating viruses are typically weak immunogens, leading to efforts to target the vaccine to mature dendritic cells (DCs). We have studied a single-cycle form of HIV-1, prepared by pseudotyping envelope-defective HIV-1 plasmids with the envelope from vesicular stomatitis virus (VSV) G protein (VSV-G), to which mo...

Uncovering HIV-1-infected cells

It has been known for more than 30 years that Human Immunodeficiency Virus 1 (HIV-1) infection drives a very potent B cell response resulting in the production of anti-HIV-1 antibodies targeting several viral proteins, particularly its envelope glycoproteins (Env) which are exposed at the surface of viral particles and infected cells [1]. Enthusiasm by the generation of these anti-Env antibodie...

Dendritic cells cross-present HIV antigens from live as well as apoptotic infected CD4+ T lymphocytes.

A better understanding of the antigen presentation pathways that lead to CD8(+) T cell recognition of HIV epitopes in vivo is needed to achieve better immune control of HIV replication. Here, we show that cross-presentation of very small amounts of HIV proteins from apoptotic infected CD4(+) T lymphocytes by dendritic cells to CD8(+) T cells is much more efficient than other known HIV presentat...

Induced ribonucleic acids in cells infected with vesicular stomatitis virus.

RNA molecules labelled in BHK 2I cells following infection with vesicular stomatitis virus in the presence of Actinomycin D and 32PO 4 were examined. A complex pattern was obtained when the RN A was centrifuged in sucrose gradients, at least five radioactive peaks being found. These had sedimentation coefficients in sucrose gradients in o-I M-sodium acetate, pH 5.o, of approx. 38 S, 28 S, 20 S,...

Rna Complexes in Vesicular Stomatitis Virus Infected L-cells Studies